Rekombinanter Histone 3 Antikörper (H3K27me3)

Produktnummer ABIN7072680

Dieser Anti-Histone 3 Antikörper ist ein Kaninchen Chimeric Antikörper zur Detektion von Histone 3 in WB, IHC, IF, ELISA, IP und ICC. Geeignet für Human.

Kurzübersicht für Rekombinanter Histone 3 Antikörper (H3K27me3) (ABIN7072680)

Target: Histone 3 (H3) (Histone H3 (H3))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: BT164

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K27me3

Verwendungszweck: Anti-H3K27me3 [BT164], Rabbit IgG, kappa

Spezifität: The epitope of BT164 was mapped in the N-terminal tail of histone H3 (27-KSAPAT-32) and included the apoptosis-induced trimethylation of K27.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: BT164 is a mice-derrived lupus antibody that was established by selection on apoptotic chromatin.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: BT164 recognizes an apoptosis-induced trimethylation of K27 on N-terminal tail of histone H3 which is a target for autoantibodies in patients and mice with SLE (systemic lupus erythematosus) (van Bavel et al., 2011, pmid: 20699234). Consequently, this antibody is recommended for the detection and studying of SLE. ELISA studies demonstrated that BT164 exhibits preferential activity with the apoptotic nucleosomes in comparison with the control nucleosomes (van Bavel et al., 2011, pmid: 20699234). It can be used for the detection of apoptosis in general. Furthermore, this antibody was demonstrated to label cells with methylated histone-3 on K27 via immunofluorescence and it also immunoprecipitated chromatin with such a methylation profile, further demonstrating its ability to specifically recognize markers characteristic of SLE (van Bavel et al., 2011, pmid: 20699234).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Trimethyl-Histone H3 (Lys27), histone H3, H3K27, Trimethylated histone H3, Histone H3 trimethylated at Lysine 27, nucleosome, H3K27ME3, post translational modification, histone post-translational modification